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A scalable pipeline for highly effective genetic modification of a malaria parasite

In malaria parasites the systematic experimental validation of drug and vaccine targets by reverse genetics is constrained by the inefficiency of homologous recombination and by the difficulty of manipulating adenine and thymine (AT) rich Plasmodium DNA in E. coli. We overcome these roadblocks by de...

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Bibliografiske detaljer
Main Authors: Pfander, Claudia, Anar, Burcu, Schwach, Frank, Otto, Thomas D., Brochet, Mathieu, Volkmann, Katrin, Quail, Michael A., Pain, Arnab, Rosen, Barry, Skarnes, William, Rayner, Julian C., Billker, Oliver
Format: Artigo
Sprog:Inglês
Udgivet: 2011
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3431185/
https://ncbi.nlm.nih.gov/pubmed/22020067
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nmeth.1742
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