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A scalable pipeline for highly effective genetic modification of a malaria parasite
In malaria parasites the systematic experimental validation of drug and vaccine targets by reverse genetics is constrained by the inefficiency of homologous recombination and by the difficulty of manipulating adenine and thymine (AT) rich Plasmodium DNA in E. coli. We overcome these roadblocks by de...
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| Autores principales: | , , , , , , , , , , , |
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| Formato: | Artigo |
| Lenguaje: | Inglês |
| Publicado: |
2011
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| Materias: | |
| Acceso en línea: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3431185/ https://ncbi.nlm.nih.gov/pubmed/22020067 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nmeth.1742 |
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