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Random and cyclical deletion of large DNA segments in the genome of Pseudomonas putida

Cumulative site-directed mutagenesis is of limited suitability for the global analysis of the gene functions in the microbe's cellular network. In order to simplify and stabilize the genome of the soil bacterium Pseudomonas putida, we developed a recyclable three-step excision method based on t...

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Autors principals: Leprince, Audrey, de Lorenzo, Víctor, Völler, Petra, van Passel, Mark W J, Martins dos Santos, Vitor A P
Format: Artigo
Idioma:Inglês
Publicat: Blackwell Publishing Ltd 2012
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC3429869/
https://ncbi.nlm.nih.gov/pubmed/22429517
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1111/j.1462-2920.2012.02730.x
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