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Development of a Method for Markerless Gene Deletion in Pseudomonas putida
We developed a negative counterselection system for Pseudomonas putida based on uracil phosphoribosyltransferase (UPRTase) and sensitivity against the antimetabolite 5-fluorouracil (5-FU). We constructed a P. putida strain that is resistant to 5-FU and constructed vectors for the deletion of the sur...
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| Main Authors: | , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
American Society for Microbiology
2011
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3147467/ https://ncbi.nlm.nih.gov/pubmed/21666018 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.05055-11 |
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