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Markerless Gene Deletion with Cytosine Deaminase in Thermus thermophilus Strain HB27
We developed a counterselectable deletion system for Thermus thermophilus HB27 based on cytosine deaminase (encoded by codA) from Thermaerobacter marianensis DSM 12885 and the sensitivity of T. thermophilus HB27 to the antimetabolite 5-fluorocytosine (5-FC). The deletion vector comprises the pUC18 o...
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| Publicat a: | Appl Environ Microbiol |
|---|---|
| Autors principals: | , , , |
| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
American Society for Microbiology
2016
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4751836/ https://ncbi.nlm.nih.gov/pubmed/26655764 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.03524-15 |
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