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Markerless Gene Deletion System for Sphingomonads
Here, we suggest that natural streptomycin resistance of many sphingomonads resides within rpsL. We constructed a dominant, streptomycin-sensitive rpsL allele and demonstrated its use as a counterselection marker in several sphingomonads. An rpsL-based markerless gene deletion system was developed a...
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| Main Authors: | , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
American Society for Microbiology
2012
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3346355/ https://ncbi.nlm.nih.gov/pubmed/22427496 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.07347-11 |
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