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Random and cyclical deletion of large DNA segments in the genome of Pseudomonas putida

Cumulative site-directed mutagenesis is of limited suitability for the global analysis of the gene functions in the microbe's cellular network. In order to simplify and stabilize the genome of the soil bacterium Pseudomonas putida, we developed a recyclable three-step excision method based on t...

Deskribapen osoa

Gorde:
Xehetasun bibliografikoak
Egile Nagusiak: Leprince, Audrey, de Lorenzo, Víctor, Völler, Petra, van Passel, Mark W J, Martins dos Santos, Vitor A P
Formatua: Artigo
Hizkuntza:Inglês
Argitaratua: Blackwell Publishing Ltd 2012
Gaiak:
Sarrera elektronikoa:https://ncbi.nlm.nih.gov/pmc/articles/PMC3429869/
https://ncbi.nlm.nih.gov/pubmed/22429517
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1111/j.1462-2920.2012.02730.x
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