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Renaturation kinetics and thermal stability of DNA in aqueous solutions of formamide and urea.

This paper reports the results of a systematic study of the effects of formamide and urea on the thermal stability and renaturation kinetics of DNA. Increasing concentrations of urea in the range 0 to 8 molar lower the Tm by 2.25 degrees C per molar, and decreases the renaturation rate by approximat...

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Pubblicato in:Nucleic Acids Res
Autore principale: Hutton, J R
Natura: Artigo
Lingua:Inglês
Pubblicazione: Oxford University Press 1977
Accesso online:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC342670/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/928068/
https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/4.10.3537
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