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Renaturation kinetics and thermal stability of DNA in aqueous solutions of formamide and urea.

This paper reports the results of a systematic study of the effects of formamide and urea on the thermal stability and renaturation kinetics of DNA. Increasing concentrations of urea in the range 0 to 8 molar lower the Tm by 2.25 degrees C per molar, and decreases the renaturation rate by approximat...

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Bibliographic Details
Main Author: Hutton, J R
Format: Artigo
Language:Inglês
Published: 1977
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC342670/
https://ncbi.nlm.nih.gov/pubmed/928068
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