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Accurate Estimation of Nucleic Acids by Amplification Efficiency Dependent PCR

Accurate estimation of template - DNA or RNA by real time PCR is dependent on the amplification efficiency (F) of the reaction. The analytical equation describing the kinetics of PCR that is influenced by template re-annealing is formulated. It predicts the gradual reduction of F - from its initial...

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Autori principali: Chatterjee, Nilanjana, Banerjee, Tanmay, Datta, Santanu
Natura: Artigo
Lingua:Inglês
Pubblicazione: Public Library of Science 2012
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC3422235/
https://ncbi.nlm.nih.gov/pubmed/22912684
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0042063
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