The isolation of duplex DNA fragments containing (dG.dC) clusters by chromatography on poly(rC)-Sephadex.
Duplex DNA containing oligo(dG.dC)-rich clusters can be isolated by specific binding to poly(rC)-Sephadex. This binding, probably mediated by the formation of an oligo(dG.dC)rC+ triple helix, is optimal at pH 5 in 50% formamide, 2 M LiCl; the bound DNA is recovered by elution at pH 7.5. Using this m...
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| Published in: | Nucleic Acids Res |
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| Main Authors: | , , |
| Format: | Artigo |
| Language: | Inglês |
| Published: |
Oxford University Press
1978
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| Online Access: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC342177/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/27765/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/5.7.2471 |
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