The isolation of duplex DNA fragments containing (dG.dC) clusters by chromatography on poly(rC)-Sephadex.

Duplex DNA containing oligo(dG.dC)-rich clusters can be isolated by specific binding to poly(rC)-Sephadex. This binding, probably mediated by the formation of an oligo(dG.dC)rC+ triple helix, is optimal at pH 5 in 50% formamide, 2 M LiCl; the bound DNA is recovered by elution at pH 7.5. Using this m...

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書目詳細資料

發表在:	Nucleic Acids Res
Principais autores:	Zuidema, D, Van den Berg, F M, Flavell, R A
格式:	Artigo
語言:	Inglês
出版:	Oxford University Press 1978
在線閱讀:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC342177/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/27765/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/5.7.2471
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