The isolation of duplex DNA fragments containing (dG.dC) clusters by chromatography on poly(rC)-Sephadex.

Duplex DNA containing oligo(dG.dC)-rich clusters can be isolated by specific binding to poly(rC)-Sephadex. This binding, probably mediated by the formation of an oligo(dG.dC)rC+ triple helix, is optimal at pH 5 in 50% formamide, 2 M LiCl; the bound DNA is recovered by elution at pH 7.5. Using this m...

Volledige beschrijving

Bibliografische gegevens

Gepubliceerd in:	Nucleic Acids Res
Hoofdauteurs:	Zuidema, D, Van den Berg, F M, Flavell, R A
Formaat:	Artigo
Taal:	Inglês
Gepubliceerd in:	Oxford University Press 1978
Online toegang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC342177/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/27765/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/5.7.2471
Tags:	Voeg label toe Geen labels, Wees de eerste die dit record labelt!