The isolation of duplex DNA fragments containing (dG.dC) clusters by chromatography on poly(rC)-Sephadex.

Duplex DNA containing oligo(dG.dC)-rich clusters can be isolated by specific binding to poly(rC)-Sephadex. This binding, probably mediated by the formation of an oligo(dG.dC)rC+ triple helix, is optimal at pH 5 in 50% formamide, 2 M LiCl; the bound DNA is recovered by elution at pH 7.5. Using this m...

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Bibliographic Details
Main Authors: Zuidema, D, Van den Berg, F M, Flavell, R A
Format: Artigo
Language:Inglês
Published: 1978
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC342177/
https://ncbi.nlm.nih.gov/pubmed/27765
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