Methods for Quantification of in vivo Changes in Protein Ubiquitination following Proteasome and Deubiquitinase Inhibition

Ubiquitination plays a key role in protein degradation and signal transduction. Ubiquitin is a small protein modifier that is adducted to lysine residues by the combined function of E1, E2, and E3 enzymes and is removed by deubiquitinating enzymes. Characterization of ubiquitination sites is importa...

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Main Authors: Udeshi, Namrata D., Mani, D. R., Eisenhaure, Thomas, Mertins, Philipp, Jaffe, Jacob D., Clauser, Karl R., Hacohen, Nir, Carr, Steven A.
Formato: Artigo
Idioma:Inglês
Publicado em: The American Society for Biochemistry and Molecular Biology 2012
Assuntos:
Research
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3418844/
https://ncbi.nlm.nih.gov/pubmed/22505724
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/mcp.M111.016857
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