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Refined Preparation and Use of Anti-diglycine Remnant (K-ε-GG) Antibody Enables Routine Quantification of 10,000s of Ubiquitination Sites in Single Proteomics Experiments

Detection of endogenous ubiquitination sites by mass spectrometry has dramatically improved with the commercialization of anti-di-glycine remnant (K-ε-GG) antibodies. Here, we describe a number of improvements to the K-ε-GG enrichment workflow, including optimized antibody and peptide input requirem...

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Bibliografiske detaljer
Main Authors: Udeshi, Namrata D., Svinkina, Tanya, Mertins, Philipp, Kuhn, Eric, Mani, D. R., Qiao, Jana W., Carr, Steven A.
Format: Artigo
Sprog:Inglês
Udgivet: The American Society for Biochemistry and Molecular Biology 2013
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3591673/
https://ncbi.nlm.nih.gov/pubmed/23266961
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/mcp.O112.027094
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