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Quantitative Assessment of Chromatin Immunoprecipitation Grade Antibodies Directed against Histone Modifications Reveals Patterns of Co-occurring Marks on Histone Protein Molecules

The defining step in most chromatin immunoprecipitation (ChIP) assays is the use of an antibody to enrich for a particular protein or histone modification state associated with segments of chromatin. The specificity of the antibody is critical to the interpretation of the experiment, yet this proper...

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Detalhes bibliográficos
Main Authors: Peach, Sally E., Rudomin, Emily L., Udeshi, Namrata D., Carr, Steven A., Jaffe, Jacob D.
Formato: Artigo
Idioma:Inglês
Publicado em: The American Society for Biochemistry and Molecular Biology 2012
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3418838/
https://ncbi.nlm.nih.gov/pubmed/22442256
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/mcp.M111.015941
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