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Protocol: Chromatin immunoprecipitation (ChIP) methodology to investigate histone modifications in two model diatom species

In this report we describe a chromatin immunoprecipitation (ChIP) protocol for two fully sequenced model diatom species Phaeodactylum tricornutum and Thalassiosira pseudonana. This protocol allows the extraction of satisfactory amounts of chromatin and gives reproducible results. We coupled the ChIP...

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Hlavní autoři: Lin, Xin, Tirichine, Leïla, Bowler, Chris
Médium: Artigo
Jazyk:Inglês
Vydáno: BioMed Central 2012
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC3546051/
https://ncbi.nlm.nih.gov/pubmed/23217141
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1746-4811-8-48
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