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Negative Selection Assay Based on Stimulation of T Cell Receptor Transgenic Thymocytes with Peptide-MHC Tetramers

Thymocyte negative selection is a requirement for the development of self tolerance. Although it is possible to assay the induction of cell death in thymocytes in vitro using antibody cross-linking, this stimulus is much stronger than the normal range of T cell receptor ligands that could be encount...

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Bibliographic Details
Main Authors: Rybakin, Vasily, Gascoigne, Nicholas R. J.
Format: Artigo
Language:Inglês
Published: Public Library of Science 2012
Subjects:
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC3416795/
https://ncbi.nlm.nih.gov/pubmed/22900100
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0043191
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