Sensitive Quantification of Clostridium difficile Cells by Reverse Transcription-Quantitative PCR Targeting rRNA Molecules

We established a sensitive and accurate quantification system for Clostridium difficile in human intestines, based on rRNA-targeted reverse transcription-quantitative PCR (RT-qPCR). We newly developed a species-specific primer set for C. difficile targeting 23S rRNA gene sequences. Both the vegetati...

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Detalhes bibliográficos
Main Authors: Matsuda, Kazunori, Tsuji, Hirokazu, Asahara, Takashi, Takahashi, Takuya, Kubota, Hiroyuki, Nagata, Satoru, Yamashiro, Yuichiro, Nomoto, Koji
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2012
Assuntos:
Microbial Ecology
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3416433/
https://ncbi.nlm.nih.gov/pubmed/22582062
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.07990-11
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