Detection of Human Intestinal Catalase-Negative, Gram-Positive Cocci by rRNA-Targeted Reverse Transcription-PCR

An analytical system based on rRNA-targeted reverse transcription-quantitative PCR (RT-qPCR) for enumeration of catalase-negative, Gram-positive cocci was established. Subgroup- or species-specific primer sets targeting 16S or 23S rRNA from Enterococcus, Streptococcus, and Lactococcus were newly dev...

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Bibliographic Details
Main Authors: Kubota, Hiroyuki, Tsuji, Hirokazu, Matsuda, Kazunori, Kurakawa, Takashi, Asahara, Takashi, Nomoto, Koji
Format: Artigo
Language:Inglês
Published: American Society for Microbiology (ASM) 2010
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Methods
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC2918946/
https://ncbi.nlm.nih.gov/pubmed/20581195
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.03132-09
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