Establishment of an Analytical System for the Human Fecal Microbiota, Based on Reverse Transcription-Quantitative PCR Targeting of Multicopy rRNA Molecules

An analytical system based on rRNA-targeted reverse transcription-quantitative PCR (RT-qPCR) was established for the precise evaluation of human intestinal microbiota. Group- and species-specific primer sets for Clostridium perfringens, Lactobacillus spp. (six subgroups and three species), Enterococ...

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Bibliographic Details
Main Authors: Matsuda, Kazunori, Tsuji, Hirokazu, Asahara, Takashi, Matsumoto, Kazumasa, Takada, Toshihiko, Nomoto, Koji
Format: Artigo
Language:Inglês
Published: American Society for Microbiology (ASM) 2009
Subjects:
Microbial Ecology
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC2663197/
https://ncbi.nlm.nih.gov/pubmed/19201979
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.01843-08
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