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Mammalian Fatty Acid Synthase Activity From Crude Tissue Lysates Tracing [(13)C]-Substrates Using GC Mass Spectrometry
Fatty Acid Synthase (FASN, FAS; EC 2.3.1.85) is the sole mammalian enzyme to synthesize fatty acids de novo from acetyl and malonyl coenzyme A esters. A new method is described that directly quantifies uniformly labeled [(13)C](16)-palmitate by tracing [(13)C](2)-acetyl-CoA and [(13)C](3)-malonyl-Co...
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| Autori principali: | , , , , , |
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| Natura: | Artigo |
| Lingua: | Inglês |
| Pubblicazione: |
2012
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| Soggetti: | |
| Accesso online: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3415257/ https://ncbi.nlm.nih.gov/pubmed/22728958 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ab.2012.06.013 |
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