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Native mass spectrometry of recombinant proteins from crude cell lysates

Determining the properties of proteins prior to purification saves time and labor. Here, we demonstrate a native mass spectrometry approach for rapid characterization of overexpressed proteins directly in crude cell lysates. The method provides immediate information on the identity, solubility, olig...

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Bibliografiske detaljer
Udgivet i:Anal Chem
Main Authors: Gan, Jinrui, Ben-Nissan, Gili, Arkind, Galina, Tarnavsky, Mark, Trudeau, Devin, Noda Garcia, Lianet, Tawfik, Dan S., Sharon, Michal
Format: Artigo
Sprog:Inglês
Udgivet: 2017
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC5702260/
https://ncbi.nlm.nih.gov/pubmed/28345863
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/acs.analchem.7b00398
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