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Monitoring the Reductive and Oxidative Half-Reactions of a Flavin-Dependent Monooxygenase using Stopped-Flow Spectrophotometry

Aspergillus fumigatus siderophore A (SidA) is an FAD-containing monooxygenase that catalyzes the hydroxylation of ornithine in the biosynthesis of hydroxamate siderophores that are essential for virulence (e.g. ferricrocin or N',N",N'''-triacetylfusarinine C)(1). The reactio...

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Autors principals: Romero, Elvira, Robinson, Reeder, Sobrado, Pablo
Format: Artigo
Idioma:Inglês
Publicat: MyJove Corporation 2012
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC3415168/
https://ncbi.nlm.nih.gov/pubmed/22453826
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/3803
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