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Fluorogenic DNA Sequencing in PDMS Microreactors
We have developed a multiplex sequencing-by-synthesis method combining terminal-phosphate labeled fluorogenic nucleotides (TPLFNs) and resealable microreactors. In the presence of phosphatase, the incorporation of a non-fluorescent TPLFN into a DNA primer by DNA polymerase results in a fluorophore....
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| Main Authors: | , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2011
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3414871/ https://ncbi.nlm.nih.gov/pubmed/21666670 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nmeth.1629 |
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