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Digital RNA sequencing minimizes sequence-dependent bias and amplification noise with optimized single-molecule barcodes
RNA sequencing (RNA-Seq) is a powerful tool for transcriptome profiling, but is hampered by sequence-dependent bias and inaccuracy at low copy numbers intrinsic to exponential PCR amplification. We developed a simple strategy for mitigating these complications, allowing truly digital RNA-Seq. Follow...
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| Main Authors: | , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
National Academy of Sciences
2012
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3268301/ https://ncbi.nlm.nih.gov/pubmed/22232676 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.1118018109 |
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