Analyzing and minimizing PCR amplification bias in Illumina sequencing libraries

Despite the ever-increasing output of Illumina sequencing data, loci with extreme base compositions are often under-represented or absent. To evaluate sources of base-composition bias, we traced genomic sequences ranging from 6% to 90% GC through the process by quantitative PCR. We identified PCR du...

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Hlavní autoři: Aird, Daniel, Ross, Michael G, Chen, Wei-Sheng, Danielsson, Maxwell, Fennell, Timothy, Russ, Carsten, Jaffe, David B, Nusbaum, Chad, Gnirke, Andreas
Médium: Artigo
Jazyk:Inglês
Vydáno: BioMed Central 2011
Témata:
Method
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC3188800/
https://ncbi.nlm.nih.gov/pubmed/21338519
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/gb-2011-12-2-r18
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