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Probing Dynein and Kinesin Stepping with Mechanical Manipulation in a Living Cell
We report a label-free assay for simultaneous optical manipulation and tracking of endogenous lipid droplets as actively transported cargoes in a living mammalian cell with sub-millisecond time resolution. Using an EM-CCD camera as a highly sensitive quadrant detector, we can detect steps of dynein-...
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| Main Authors: | , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2009
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3659426/ https://ncbi.nlm.nih.gov/pubmed/19504528 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/cphc.200900113 |
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