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The synthesis of oligonucleotides containing an aliphatic amino group at the 5' terminus: synthesis of fluorescent DNA primers for use in DNA sequence analysis.

A rapid and versatile method has been developed for the synthesis of oligonucleotides which contain an aliphatic amino group at their 5' terminus. This amino group reacts specifically with a variety of electrophiles, thereby allowing other chemical species to be attached to the oligonucleotide. This...

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Detalles Bibliográficos
Publicado en:Nucleic Acids Res
Principais autores: Smith, L M, Fung, S, Hunkapiller, M W, Hunkapiller, T J, Hood, L E
Formato: Artigo
Idioma:Inglês
Publicado: Oxford University Press 1985
Acceso en liña:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC341163/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/4000959/
https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/13.7.2399
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