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A novel strategy for constructing clustered point mutations.

We have modified the synthetic linker mutagenesis procedure (1,2) in order to facilitate both the construction and the analysis of deletions, insertions and clustered point mutations generated in DNA in vitro. The protocol as originally described by McKnight and Kingsbury (1) involved attaching a sy...

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Библиографические подробности
Опубликовано в: :Nucleic Acids Res
Главные авторы: Haltiner, M, Kempe, T, Tjian, R
Формат: Artigo
Язык:Inglês
Опубликовано: Oxford University Press 1985
Online-ссылка:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC341049/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2987803/
https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/13.3.1015
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