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Construction of bacteriophage T7 late promoters with point mutations and characterization by in vitro transcription properties.

This paper describes the construction of 18 cloned bacteriophage T7 late promoters with single point mutations. In vitro transcription experiments were used to characterize the properties of these promoters. Since the mutated promoters are cloned into identical backgrounds, differences seen in the t...

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Bibliografiske detaljer
Udgivet i:Nucleic Acids Res
Main Authors: Chapman, K A, Burgess, R R
Format: Artigo
Sprog:Inglês
Udgivet: Oxford University Press 1987
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC305969/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3299271/
https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/15.13.5413
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