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Construction of bacteriophage T7 late promoters with point mutations and characterization by in vitro transcription properties.

This paper describes the construction of 18 cloned bacteriophage T7 late promoters with single point mutations. In vitro transcription experiments were used to characterize the properties of these promoters. Since the mutated promoters are cloned into identical backgrounds, differences seen in the t...

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Detalhes bibliográficos
Main Authors: Chapman, K A, Burgess, R R
Formato: Artigo
Idioma:Inglês
Publicado em: 1987
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC305969/
https://ncbi.nlm.nih.gov/pubmed/3299271
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