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Synthetic Spike-in Standards Improve Run-Specific Systematic Error Analysis for DNA and RNA Sequencing

While the importance of random sequencing errors decreases at higher DNA or RNA sequencing depths, systematic sequencing errors (SSEs) dominate at high sequencing depths and can be difficult to distinguish from biological variants. These SSEs can cause base quality scores to underestimate the probab...

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Detalhes bibliográficos
Main Authors: Zook, Justin M., Samarov, Daniel, McDaniel, Jennifer, Sen, Shurjo K., Salit, Marc
Formato: Artigo
Idioma:Inglês
Publicado em: Public Library of Science 2012
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3409179/
https://ncbi.nlm.nih.gov/pubmed/22859977
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0041356
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