Stereospecificity of nucleases towards phosphorothioate-substituted RNA: stereochemistry of transcription by T7 RNA polymerase.

Transcription by T7 RNA polymerase has been studied using a chiral ATP analogue. The Sp diastereoisomer of adenosine 5'-O-(1-thiotriphosphate) (ATP alpha S) was incorporated into RNA with an apparent KM of approximately 15 microM, similar to that for ATP; the Rp diastereoisomer was neither a substra...

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Veröffentlicht in:	Nucleic Acids Res
Hauptverfasser:	Griffiths, A D, Potter, B V, Eperon, I C
Format:	Artigo
Sprache:	Inglês
Veröffentlicht:	Oxford University Press 1987
Online-Zugang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC340838/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2438652/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/15.10.4145
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