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Oligonucleotides complementary to a promoter over the region -8...+2 as transcription primers for E. coli RNA polymerase.

Primer-dependent transcription by E. coli RNA polymerase on T7 promoter A2 has been studied. Synthetic deoxyribonucleotides complementary to the promoter over the region -8...+2 were taken as primers. A ribonucleoside residue was present at the 3'-end of some of these oligonucleotides. The octa...

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Publicado en:Nucleic Acids Res
Autores principales: Grachev, M A, Zaychikov, E F, Ivanova, E M, Komarova, N I, Kutyavin, I V, Sidelnikova, N P, Frolova, I P
Formato: Artigo
Lenguaje:Inglês
Publicado: Oxford University Press 1984
Acceso en línea:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC320395/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6390344/
https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/12.22.8509
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