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An Efficient Method for the Long-Term and Specific Expression of Exogenous cDNAs in Cultured Purkinje Neurons
We present a simple and efficient method for expressing cDNAs in Purkinje neurons (PNs) present in heterogeneous mouse cerebellar cultures. The method combines the transfection of freshly-dissociated cerebellar cells via nucleofection with the use of novel expression plasmids containing a fragment o...
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| Main Authors: | , , |
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| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
2011
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3407467/ https://ncbi.nlm.nih.gov/pubmed/21708190 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jneumeth.2011.06.006 |
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