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An Efficient Method for the Long-Term and Specific Expression of Exogenous cDNAs in Cultured Purkinje Neurons

We present a simple and efficient method for expressing cDNAs in Purkinje neurons (PNs) present in heterogeneous mouse cerebellar cultures. The method combines the transfection of freshly-dissociated cerebellar cells via nucleofection with the use of novel expression plasmids containing a fragment o...

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Auteurs principaux: Wagner, Wolfgang, McCroskery, Seumas, Hammer, John A.
Format: Artigo
Langue:Inglês
Publié: 2011
Sujets:
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC3407467/
https://ncbi.nlm.nih.gov/pubmed/21708190
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jneumeth.2011.06.006
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