A Method for Analyzing Protein–Protein Interactions in the Plasma Membrane of Live B Cells by Fluorescence Resonance Energy Transfer Imaging as Acquired by Total Internal Reflection Fluorescence Microscopy

For more than a decade, fluorescence resonance energy transfer (FRET) imaging methods have been developed to study dynamic interactions between molecules at the nanometer scale in live cells. Here, we describe a protocol to measure FRET by the acceptor-sensitized emission method as detected by total...

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Main Authors: Sohn, Hae Won, Tolar, Pavel, Brzostowski, Joseph, Pierce, Susan K.
Format: Artigo
Sprog:Inglês
Udgivet: 2010
Fag:
Article
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3406930/
https://ncbi.nlm.nih.gov/pubmed/19957130
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-60761-404-3_10
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