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In vitro construction of yeast tRNAAsp variants: nucleotide substitutions and additions in T-stem and T-loop.

A procedure for the construction of 3'-end labelled yeast tRNAAsp harboring substitutions or additions of any desired nucleotide in T-stem and T-loop (position 57 to 61) has been developed. This was done by in vitro enzymatic manipulations of the yeast tRNAAsp involving specific hydrolysis with RNas...

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Bibliografske podrobnosti
izdano v:Nucleic Acids Res
Principais autores: Carbon, P, Ebel, J P
Format: Artigo
Jezik:Inglês
Izdano: Oxford University Press 1987
Online dostop:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC340609/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3550694/
https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/15.5.1933
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