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In vitro construction of yeast tRNAAsp variants: nucleotide substitutions and additions in T-stem and T-loop.
A procedure for the construction of 3'-end labelled yeast tRNAAsp harboring substitutions or additions of any desired nucleotide in T-stem and T-loop (position 57 to 61) has been developed. This was done by in vitro enzymatic manipulations of the yeast tRNAAsp involving specific hydrolysis with...
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| Main Authors: | , |
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| Format: | Artigo |
| Language: | Inglês |
| Published: |
1987
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| Online Access: | https://ncbi.nlm.nih.gov/pmc/articles/PMC340609/ https://ncbi.nlm.nih.gov/pubmed/3550694 |
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