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In vitro construction of yeast tRNAAsp variants: nucleotide substitutions and additions in T-stem and T-loop.

A procedure for the construction of 3'-end labelled yeast tRNAAsp harboring substitutions or additions of any desired nucleotide in T-stem and T-loop (position 57 to 61) has been developed. This was done by in vitro enzymatic manipulations of the yeast tRNAAsp involving specific hydrolysis with...

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Autori principali: Carbon, P, Ebel, J P
Natura: Artigo
Lingua:Inglês
Pubblicazione: 1987
Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC340609/
https://ncbi.nlm.nih.gov/pubmed/3550694
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