In vitro construction of yeast tRNAAsp variants: nucleotide substitutions and additions in T-stem and T-loop.
A procedure for the construction of 3'-end labelled yeast tRNAAsp harboring substitutions or additions of any desired nucleotide in T-stem and T-loop (position 57 to 61) has been developed. This was done by in vitro enzymatic manipulations of the yeast tRNAAsp involving specific hydrolysis with RNas...
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| Veröffentlicht in: | Nucleic Acids Res |
|---|---|
| Hauptverfasser: | , |
| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
Oxford University Press
1987
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| Online-Zugang: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC340609/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3550694/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/15.5.1933 |
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