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Highly selective chemical modification of cruciform loops by diethyl pyrocarbonate.
Diethyl pyrocarbonate reacts with the single-stranded loops of cruciform structures with great selectivity. Adenine bases are carbethoxylated, as a result of which the backbone may be cleaved with piperidine, and the level of chemical modification at each base may be determined. We have studied the...
Uloženo v:
| Hlavní autoři: | , |
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| Médium: | Artigo |
| Jazyk: | Inglês |
| Vydáno: |
1986
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| On-line přístup: | https://ncbi.nlm.nih.gov/pmc/articles/PMC339841/ https://ncbi.nlm.nih.gov/pubmed/3012460 |
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