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Highly selective chemical modification of cruciform loops by diethyl pyrocarbonate.

Diethyl pyrocarbonate reacts with the single-stranded loops of cruciform structures with great selectivity. Adenine bases are carbethoxylated, as a result of which the backbone may be cleaved with piperidine, and the level of chemical modification at each base may be determined. We have studied the...

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Pubblicato in:Nucleic Acids Res
Autori principali: Furlong, J C, Lilley, D M
Natura: Artigo
Lingua:Inglês
Pubblicazione: Oxford University Press 1986
Accesso online:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC339841/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3012460/
https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/14.10.3995
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