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Structure- and sequence-specificity of ozone degradation of supercoiled plasmid DNA.
Ozone-reactive sites on the nucleobase moieties in supercoiled pBR322 DNA were investigated by using sequencing procedures. Ozonolysis in the absence of salt resulted in degradation of thymine residues in the A + T rich region located at 3100-3400bp. In the presence of salt, such as NaCl or MgCl2, a...
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| Main Authors: | , , , , , |
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| Format: | Artigo |
| Language: | Inglês |
| Published: |
1986
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| Online Access: | https://ncbi.nlm.nih.gov/pmc/articles/PMC339495/ https://ncbi.nlm.nih.gov/pubmed/3005966 |
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