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Structure- and sequence-specificity of ozone degradation of supercoiled plasmid DNA.

Ozone-reactive sites on the nucleobase moieties in supercoiled pBR322 DNA were investigated by using sequencing procedures. Ozonolysis in the absence of salt resulted in degradation of thymine residues in the A + T rich region located at 3100-3400bp. In the presence of salt, such as NaCl or MgCl2, a...

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Detalhes bibliográficos
Main Authors: Sawadaishi, K, Miura, K, Ohtsuka, E, Ueda, T, Shinriki, N, Ishizaki, K
Formato: Artigo
Idioma:Inglês
Publicado em: 1986
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC339495/
https://ncbi.nlm.nih.gov/pubmed/3005966
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