Zinc-finger nuclease-mediated targeted insertion of reporter genes for quantitative imaging of gene expression in sea urchin embryos

To understand complex biological systems, such as the development of multicellular organisms, it is important to characterize the gene expression dynamics. However, there is currently no universal technique for targeted insertion of reporter genes and quantitative imaging in multicellular model syst...

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Bibliographische Detailangaben
Hauptverfasser: Ochiai, Hiroshi, Sakamoto, Naoaki, Fujita, Kazumasa, Nishikawa, Masatoshi, Suzuki, Ken-ichi, Matsuura, Shinya, Miyamoto, Tatsuo, Sakuma, Tetsushi, Shibata, Tatsuo, Yamamoto, Takashi
Format: Artigo
Sprache:Inglês
Veröffentlicht: National Academy of Sciences 2012
Schlagworte:
Biological Sciences
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3390856/
https://ncbi.nlm.nih.gov/pubmed/22711830
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.1202768109
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