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Unexpected tautomeric equilibria of the carbanion-enamine intermediate in pyruvate oxidase highlight unrecognized chemical versatility of thiamin

Thiamin diphosphate, the vitamin B1 coenzyme, plays critical roles in fundamental metabolic pathways that require acyl carbanion equivalents. Studies on chemical models and enzymes had suggested that these carbanions are resonance-stabilized as enamines. A crystal structure of this intermediate in p...

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Bibliografske podrobnosti
Main Authors: Meyer, Danilo, Neumann, Piotr, Koers, Eline, Sjuts, Hanno, Lüdtke, Stefan, Sheldrick, George M., Ficner, Ralf, Tittmann, Kai
Format: Artigo
Jezik:Inglês
Izdano: National Academy of Sciences 2012
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC3390845/
https://ncbi.nlm.nih.gov/pubmed/22730460
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.1201280109
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