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Solid supported hydrolysis of apurinic sites in synthetic oligonucleotides for rapid and efficient purification on reverse-phase cartridges.

Rapid purification methods for synthetic oligonucleotides involving reverse-phase cartridges (RPC) and enzymatic hydrolysis have been introduced. These methods are based on a discrimination between the desired target fragment protected with a 5'-DMT group and incompletely elongated products possessi...

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Detalles Bibliográficos
Publicado en:Nucleic Acids Res
Principais autores: Horn, T, Urdea, M S
Formato: Artigo
Idioma:Inglês
Publicado: Oxford University Press 1988
Acceso en liña:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC339077/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3145494/
https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/16.24.11559
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