PCR Amplification and Transcription for Site-Specific Labeling of Large RNA Molecules by a Two-Unnatural-Base-Pair System

For the site-specific labeling and modification of RNA by genetic alphabet expansion, we developed a PCR and transcription system using two hydrophobic unnatural base pairs: 7-(2-thienyl)-imidazo[4,5-b]pyridine (Ds) and 2-nitro-4-propynylpyrrole (Px) as a third pair for PCR amplification and Ds and...

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Hauptverfasser: Kimoto, Michiko, Yamashige, Rie, Yokoyama, Shigeyuki, Hirao, Ichiro
Format: Artigo
Sprache:Inglês
Veröffentlicht: Hindawi Publishing Corporation 2012
Schlagworte:
Research Article
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3390107/
https://ncbi.nlm.nih.gov/pubmed/22792445
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1155/2012/230943
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