Highly specific unnatural base pair systems as a third base pair for PCR amplification

Toward the expansion of the genetic alphabet of DNA, we present highly efficient unnatural base pair systems as an artificial third base pair for PCR. Hydrophobic unnatural base pair systems between 7-(2-thienyl)imidazo[4,5-b]pyridine (Ds) and 2-nitro-4-propynylpyrrole (Px) were fine-tuned for effic...

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Bibliografiske detaljer
Main Authors: Yamashige, Rie, Kimoto, Michiko, Takezawa, Yusuke, Sato, Akira, Mitsui, Tsuneo, Yokoyama, Shigeyuki, Hirao, Ichiro
Format: Artigo
Sprog:Inglês
Udgivet: Oxford University Press 2012
Fag:
Synthetic Biology and Chemistry
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3315302/
https://ncbi.nlm.nih.gov/pubmed/22121213
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkr1068
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