Highly specific unnatural base pair systems as a third base pair for PCR amplification

Toward the expansion of the genetic alphabet of DNA, we present highly efficient unnatural base pair systems as an artificial third base pair for PCR. Hydrophobic unnatural base pair systems between 7-(2-thienyl)imidazo[4,5-b]pyridine (Ds) and 2-nitro-4-propynylpyrrole (Px) were fine-tuned for effic...

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Detalhes bibliográficos
Main Authors: Yamashige, Rie, Kimoto, Michiko, Takezawa, Yusuke, Sato, Akira, Mitsui, Tsuneo, Yokoyama, Shigeyuki, Hirao, Ichiro
Formato: Artigo
Idioma:Inglês
Publicado em: Oxford University Press 2012
Assuntos:
Synthetic Biology and Chemistry
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3315302/
https://ncbi.nlm.nih.gov/pubmed/22121213
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkr1068
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