Highly specific unnatural base pair systems as a third base pair for PCR amplification

Toward the expansion of the genetic alphabet of DNA, we present highly efficient unnatural base pair systems as an artificial third base pair for PCR. Hydrophobic unnatural base pair systems between 7-(2-thienyl)imidazo[4,5-b]pyridine (Ds) and 2-nitro-4-propynylpyrrole (Px) were fine-tuned for effic...

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Hlavní autoři: Yamashige, Rie, Kimoto, Michiko, Takezawa, Yusuke, Sato, Akira, Mitsui, Tsuneo, Yokoyama, Shigeyuki, Hirao, Ichiro
Médium: Artigo
Jazyk:Inglês
Vydáno: Oxford University Press 2012
Témata:
Synthetic Biology and Chemistry
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC3315302/
https://ncbi.nlm.nih.gov/pubmed/22121213
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkr1068
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