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Highly specific unnatural base pair systems as a third base pair for PCR amplification

Toward the expansion of the genetic alphabet of DNA, we present highly efficient unnatural base pair systems as an artificial third base pair for PCR. Hydrophobic unnatural base pair systems between 7-(2-thienyl)imidazo[4,5-b]pyridine (Ds) and 2-nitro-4-propynylpyrrole (Px) were fine-tuned for effic...

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Bibliografske podrobnosti
Main Authors: Yamashige, Rie, Kimoto, Michiko, Takezawa, Yusuke, Sato, Akira, Mitsui, Tsuneo, Yokoyama, Shigeyuki, Hirao, Ichiro
Format: Artigo
Jezik:Inglês
Izdano: Oxford University Press 2012
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC3315302/
https://ncbi.nlm.nih.gov/pubmed/22121213
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkr1068
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